Reconstituting a lyophilized peptide means adding bacteriostatic water to the vial before laboratory use. Enter the vial size, the volume of water you plan to add, and a reference amount, and this free peptide reconstitution calculator gives the resulting concentration in mg/mL, the draw volume, and the corresponding volume in microliters, along with total draws per vial.
How long will this vial last?
Reconstitution questions
How is peptide concentration calculated?
Concentration is the amount of peptide divided by the volume of bacteriostatic water added. A 10 mg vial reconstituted with 2 mL of water yields 5 mg/mL.
What draw volume does the calculator report?
The draw volume in milliliters (mL), with the same figure in microliters (1 mL = 1000 µL). It is the amount of reconstituted solution that contains the reference amount you entered.
Does adding more bacteriostatic water change the amount of peptide?
No. Adding more water lowers the concentration, so each milliliter contains less peptide, but the total amount in the vial is unchanged. More water simply means larger draw volumes for the same amount.
How do you calculate the draw volume for 250 mcg?
Convert micrograms to milligrams first: 250 mcg is 0.25 mg. Divide 0.25 mg by the concentration in mg/mL to get the draw volume in mL. At 5 mg/mL that is 0.05 mL, or 50 µL.
How should a reconstituted vial be stored?
Reconstituted research peptides are typically refrigerated at 36-46 degrees F, protected from light, and used within 60 days. Lyophilized (unreconstituted) vials keep considerably longer.
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All information and products are for laboratory research use only and are not for human or animal consumption. This tool is a unit converter, not medical or dosing advice.