Reconstituting a lyophilized peptide means adding bacteriostatic water to the vial before laboratory use. Enter the vial size, the volume of water you plan to add, and a reference amount, and this free peptide reconstitution calculator gives the resulting concentration in mg/mL, the draw volume, and the equivalent units on a standard U-100 insulin syringe, along with total doses per vial.
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Reconstitution questions
How is peptide concentration calculated?
Concentration is the amount of peptide divided by the volume of bacteriostatic water added. A 10 mg vial reconstituted with 2 mL of water yields 5 mg/mL.
What do units mean on an insulin syringe?
A standard U-100 insulin syringe divides 1 mL into 100 units, so each unit is 0.01 mL. The calculator converts a draw volume into those units for convenience.
Does adding more bacteriostatic water change the dose?
No. Adding more water lowers the concentration, so each syringe unit contains less peptide, but the total amount in the vial is unchanged. More water simply means larger draw volumes for the same reference amount.
How do you calculate a 250 mcg dose?
Convert micrograms to milligrams first: 250 mcg is 0.25 mg. Divide 0.25 mg by the concentration in mg/mL to get the draw volume, then multiply by 100 for U-100 syringe units. At 5 mg/mL, 250 mcg is 0.05 mL, or 5 units.
How should a reconstituted vial be stored?
Reconstituted research peptides are typically refrigerated at 36-46 degrees F, protected from light, and used within 60 days. Lyophilized (unreconstituted) vials keep considerably longer.
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All information and products are for laboratory research use only and are not for human or animal consumption. This tool is a unit converter, not medical or dosing advice.